Scheme :

	Max. Marks	Min. Pass Marks
Paper-I	40
Paper-II	40
Practicals	120

Paper-I
BIO-CHEMISTRY AND SYSTEMIC BACTERIOLOGY

Max. Marks : 40 Section-A Bio-Chemistry-II

1. Blood sugar and its regulation.
2. Chemical examination of urine.
3. Glucose tolerance test.
4. Diabetes mellitus, Ketosis, Diabetic coma.
5. Non-protein nitrogenous compounds.
6. The Plasma Proteins and A.G. Ratio.
7. Lipids.
8. Enzymes.
9. Tests of Gastric Function. Occulat Blood.
10. Test in Liver and Biliary Tract Disease.
11. Tests in Pancreatic Disease, Steatorrhoea.
12. Acid Base Regulation.
13. Basal Metabolism Blood Oxygen.
14. Regulation of pH in blood and body fulids.
15. Vitamins and their tests.
16. Chemical Examination of Cerebrospinal fluid.
17. Milk Analysis.
18. Stones (Urinary and Gallbladder).
19. Faecal Pigments.
20. Drugs and Poisons, Alcohol.
21. Water management and its analysis.

Section-B Systemic Bacteriology and other microbes

1. Classification of bacteria.
2. Normal bacterial flora and its importance.
3. Introduction and Laboratory methods of identification of :
   1. Staphylococci
   2. Streptococci
   3. Pneumococci
   4. Neisseria
   5. Diphtheria
   6. Anthrax
   7. Clostridia
   8. Anteric Gram Negative Rod
   9. Cholera
   10. Pseducomonas
   11. Haemophilia's and Borde-tella
   12. Spirochetes
   13. Spirillum and Leptospira
   14. Ricetsial and Chalmydia
4. Mycology : General characters of Fungi : Yeast like fungi and their diseases; Dermatophytoses.
5. Virology : General characters and classification, Diagnostic methods, materials collected for virus isolation and preservation of material.
6. Bacteriology of water, milk and food.

Paper-II
SEROLOGY AND GENERAL PATHOLOGY

Max. Marks : 40 Section-A Serology :

1. Antigens and antibodies.
2. Complement and fixation test.
3. Agglutination.
4. Precipitation.
5. Flocculation.
6. Neutralization.
7. Immunofluorescence.
8. Immune electrophoresis.
9. Serology of Syphillis.
10. Haem Agglutinated Haem Aggulution Inhibition.
11. Skin Tests

Section-B General Pathology

1. Introduction to pathology (Morbid Anatomy and Histology)
2. Degeneration, Necrosis and gangarene and how to recognise them.
3. Infection, Thrombosis and Embolism
4. Inflammation.
5. Neopiasia, Benign and Melignant tumors.

PRACTICALS

Practicals

(75 *3 periods)

Max. Marks : 120

Unit-I Bio-Chemistry

1. Cholesterol, Triglycorides, H.D.L., L.D.L.
2. Enzymes, S.G.O.T., S.G.P.T.,L.D.H.
3. Alkaline and Acid phosphatase.
4. F.T.M. Gastric analysis.
5. Serum Electrolytes.
6. Principle and application of spectrophotometer, Turbindimeter and chromatography.

Unit-II A. Haematology :

1. Erythrocyte Sedimentation Rate (E.S.R.) and Packed Cell volume (P.C.V.) and Total R.B.C. count.
2. Red cell indices and their calculation.
3. Staining of Reticulocyte and their counting.
4. Total Eosinophil Count.
5. Cyto chemical stains used in bone Marrow and P.B.f. for diagnosis.
6. Blood Grouping and Rh typing and cross matching.
7. Anti "D" Titresw in Maternal blood.
8. Coomb's Test.
9. Demonstrations of collection of Blood for transfusion.

B. Serology and Bacteriology :

1. Cleaning and sterilisation.
2. Widal Test.
3. Brucella Aggultination.
4. Alberts Stain and Neisser Stain.
5. A.F.B. Stain
6. Concentration method of A.F.B.
7. Smears of Leprosy (Nasal Smear Slit Smear).
8. Staining and examination of prepared smears of Pus, Sputum conjunctive etc. by Gram A.F.B. (R.N. Method)
9. Demonstration of instruments for sterilization.
10. Composition of common laboratory cultures media.
11. Demonstration of some common types of growth of micro organism on different media.
12. Prepration and staining for smears from cultures.
13. Demonstration of special biochemical tests used in identification of organisism.
14. Collection of uring for culture from females, males and children, taking of throats swabs/conjunctival swabs.

C. Cytology and Histology :

1. Microscopy of urine and C.S.F.
2. Semen examination for sperms count and motility.
3. Demonstration of the paraffin embedding, sectioning for histopathology.
4. Himotoxyline and eosine staining of paraffin section and taking and staining of smears for sex chromation.
5. Making and fixing of smears from urine, sputum and Vaginal etc. for cytology.
6. Staining of smears for exfoliative cytology.